Part:BBa_K325229

Green Firefly Luciferase and LRE
L. Cruciata
(E. coli optimised)

Input: L-Arabinose Output: Light (green)

pBad/araC I0500 mutated version of Luciferase/LRE K325210

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Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Val239Ile mutation was used to create a green bioluminescence.

Colour of emission

Figure 1 - Comparison of part BBa_K325229 against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3

Sequences

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 3044
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  Illegal NheI site found at 3278
  Illegal SpeI site found at 3044
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  Illegal BamHI site found at 2169
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 3044
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 3044
  Illegal AgeI site found at 979
  Illegal AgeI site found at 2118
  Illegal AgeI site found at 3820
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  Illegal SapI.rc site found at 1550
  Illegal SapI.rc site found at 3334